In response to high oil prices in the past, Brazil’s total annual sugar cane production increased from 79.8 million tons in 1970 to 620 million tons in 2018-2019, and due to increased market demand, several new sugar cane plants were built from 2004 onwards, reaching 343 units in 2019. The potential of biogas production from the production residues of the Brazilian sugar and alcohol industry (vinasse, filter cake, bagasse and straw) has already been demonstrated in several studies. In the biogas production process, one of the technical challenges is the nutrient input of the plants, where several aspects, such as